Table 1.
Arylsulfatase activity due to expression of the flaB::astA transcriptional fusion in C. jejuni DRH665 non-motile variants and associated in vitro-isolated revertants.a
| Strainb | Arylsulfatase Units |
|---|---|
| DRH665 (Wt) | 246.59 ± 6.06 |
| DRH939 (ΔflgS) | < 0.13 ± 0.05c |
| DRH665 5W2 | 0.23 ± 0.13 |
| DRH665 5W6 | 0.37 ± 0.10 |
| DRH665 10W1 | 0.31 ± 0.03 |
| in vitro-isolated revertants | |
| DRH665 5W2 rev1 | 221.09 ± 6.06 |
| DRH665 5W2 rev5 | 246.28 ± 7.42 |
| DRH665 5W6 rev3 | 225.40 ± 4.07 |
| DRH665 5W6 rev4 | 244.58 ± 11.31 |
| DRH665 5W6 rev5 | 216.76 ± 17.30 |
| DRH665 5W6 rev7A | 240.35 ± 2.70 |
| DRH665 5W6 rev7B | 196.27 ± 8.15 |
| DRH665 5W6 rev8 | 228.52 ± 7.49 |
| DRH665 10W1 rev3 | 337.56 ± 6.16 |
| DRH665 10W1 rev4 | 249.96 ± 7.54 |
a
Results are from a typical assay with each sample performed in triplicate. Values are reported as arylsulfatase units. One unit equals the amount of arylsulfatase required to generate 1 nmol of nitrophenol per hour per OD600 1.0.
b
Strains used include DRH665 (81–176 SmR ΔastA flaB::astA), DRH939 (81–176 SmR ΔastA ΔflgS flaB::astA), and the non-motile variants DRH665 5W2, DRH665 5W6, and DRH665 10W1 and their associated in vitro-isolated revertants.
c
At least one of the three tested samples had a value below the limit of detection of the assay which was 0.6 arylsulfatase units.