Table 2.
Arylsulfatase activity due to expression from the flaB::astA transcriptional fusion in C. jejuni DRH665 non-motile variants and associated in vivo-isolated revertants.a
| Strainb | Arylsulfatase Units |
|---|---|
| DRH665 (Wt) | 251.02 ± 17.95 |
| DRH939 (ΔflgS) | < 0.09 ± 0.06c |
| DRH665 5W2 | 0.32 ± 0.14 |
| DRH665 5W6 | 0.22 ± 0.16 |
| in vivo-isolated revertants | |
| DRH665 5W2 rev8 | 66.21 ± 9.87 |
| DRH665 5W2 rev9 | 64.78 ± 8.78 |
| DRH665 5W2 rev10 | 70.32 ± 6.69 |
| DRH665 5W6 rev10 | 82.75 ± 15.41 |
a
Results are from a typical assay with each sample performed in triplicate. Values are reported as arylsulfatase units. One unit equals the amount of arylsulfatase required to generate 1 nmol of nitrophenol per hour per OD600 1.0.
b
Strains used include DRH665 (81–176 SmR ΔastA flaB::astA), DRH939 (81–176 SmR ΔastA ΔflgS flaB::astA), and the non-motile variants DRH665 5W2 and DRH665 5W6, and their associated in vivo-isolated revertants.
c
At least one of the three tested samples had a value below the limit of detection of the assay which was 0.6 arylsulfatase units.