Table 3.
Analysis of flagellar number produced by C. jejuni strains.
| Percentage of bacteria withd | |||||
|---|---|---|---|---|---|
| Straina | FlgSb | FlgRc | 2 flagella | 1 flagellum | 0 flagella |
| DRH665 | Wt | Wt | 48.4 | 41.8 | 9.8 |
| DRH665 5W2 rev8 | FlgS 5W2 | FlgR R382K | 0 | 17.1 | 82.8 |
| DRH665 5W2 rev9 | FlgS 5W2 | FlgR R382K | 1.6 | 10.9 | 87.5 |
| DRH665 5W2 rev10 | FlgS 5W2 | FlgR R382K | 0 | 11.9 | 88.1 |
| DRH665 5W6 rev10 | FlgS 5W6 | FlgR R382K | 1.6 | 17.1 | 81.3 |
| DRH460 | Nonee | Wt | 0 | 0 | 100 |
| DRH2853 | Nonee | FlgR R382Kg | 1.6 | 9.5 | 88.9 |
| DRH2724 | FlgS 5W6f | Wt | 0 | 0 | 100 |
| DRH2861 | FlgS 5W6f | FlgR R382Kg | 0.8 | 10.6 | 88.7 |
Strains used include DRH665 (81–176 SmR ΔastA flaB::astA), the in vivo-isolated revertants DRH665 5W2 rev8, rev9, and rev10 and DRH665 5W6 rev10, DRH460 (81–176 SmR ΔflgS), DRH2853 (81–176 SmR ΔflgS flgR5W6rev10), DRH2724 (81–176 SmR ΔastA ΔflgS flaB::astA), and DRH2861 (81–176 SmR ΔastA ΔflgS flgR5W6rev10 flaB::astA).
FlgS protein produced in each strain
FlgR protein produced in each strain
Over 120 individual bacteria for each strain were examined to determine the percentage of bacteria producing each number of flagella.
Strains contain an in-frame deletion of the flgS coding sequence and do not produce FlgS.
Wild-type flgS has been replaced with the flgS allele from DRH665 5W6.
Wild-type flgR has been replaced with the flgR allele from DRH665 5W6 rev10.