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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Mol Microbiol. 2008 Aug 29;70(2):519–536. doi: 10.1111/j.1365-2958.2008.06428.x

Table 4.

Arylsulfatase activity due to expression of the flaB::astA transcriptional fusion in flgS and flgR mutants of in vivo-isolated revertants of C. jejuni DRH665 5W2 and DRH665 5W6.a

Arylsulfatase Units
In vitro-isolated Revertant No Mutationb flgS::cat-rpsLc flgR::cat-rpsLd
DRH665 5W2 rev8 61.38 ± 4.45 44.30 ± 3.02 2.43 ± 1.33
DRH665 5W2 rev9 67.99 ± 5.18 70.23 ± 6.13 0.10 ± 0.07
DRH665 5W2 rev10 62.59 ± 4.67 66.11 ± 4.26 0.24 ± 0.14
DRH665 5W6 rev10 60.97 ± 19.39 44.36 ± 6.89 < 0.2 ± 0.14
a

Results are from a typical assay with each sample performed in triplicate. Values are reported as arylsulfatase units. One unit equals the amount of arylsulfatase required to generate 1 nmol of nitrophenol per hour per OD600 1.0.

b

Strains contain the flgS and flgR alleles associated with the original in vitro-isolated revertants.

c

The flgS allele of each revertant has been replaced with flgS::cat-rpsL to eliminate FlgS production.

d

The flgR allele of each revertant has been replaced with flgR::cat-rpsL to eliminate FlgR production.