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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Mol Microbiol. 2008 Aug 29;70(2):519–536. doi: 10.1111/j.1365-2958.2008.06428.x

Table 5.

Arylsulfatase activity due to expression of the flaB::astA transcriptional fusion in flgS5W6 mutants containing wild-type flgR or flgR5W6rev10.a

Strainb flgS allelec flgR alleled Arylsulfatase Units
DRH665 Wt Wt 221.37 ± 11.67
DRH939 Deletion Wt 0.44 ± 0.05
DRH2737 flgS5W6 Wt < 0.08 ± 0.03e
DRH2874 flgS5W6 flgR5W6rev10 37.53 ± 4.42
a

Results are from a typical assay with each sample performed in triplicate. Values are reported as arylsulfatase units. One unit equals the amount of arylsulfatase required to generate 1 nmol of nitrophenol per hour per OD600 1.0.

b

Strains used include DRH665 (81–176 SmR ΔastA flaB::astA), DRH939 (81–176 SmR ΔastA ΔflgS flaB::astA), DRH2737 (81–176 SmR ΔastA flgS5W6 flaB::astA), and DRH2874 (81–176 SmR ΔastA flgS5W6 flgR5W6rev10).

c

The type of flgS allele contained in each strain. flgS has been removed in DRH939.

d

The type of flgR allele contained in each strain. DRH2874 has had flgR replaced with the flgR allele of DRH665 5W6 rev10.

e

At least one of the three tested samples had a value below the limit of detection of the assay which was 0.06 arylsulfatase units.