FIGURE 1.

Successful ablation of MAT-IIβ subunit using viral encoded shRNA. MAT-II α2 and β protein expression (60 μg of protein/well) and mRNA levels were analyzed in untransduced or ≥98% transduced cells as detailed under “Experimental Procedures.” Quantitative real time PCR was conducted using RNA extracted from cells maintained in 100 μm l-Met RPMI medium, and RNA copy number was calculated as detailed under “Experimental Procedures.” The results (means ± S.E.) of two combined experiments, each done in triplicate, are shown. Statistical differences were determined as described under “Experimental Procedures.” MAT-IIβ expression was significantly ablated in V1110 cells. ^***, p ≤ 0.001. MAT-IIα2 was slightly elevated or unaffected (p > 0.05).