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AG1478 treatment causes Golgi fragmentation in H4 cells. H4-p58 cells (A) or H4 cells (C) were treated with DMSO or 14 μm AG1478 as indicated for 1 h. H4 cells were fixed and stained with anti-GM130 antibody. B, chemical structures of AG1478 and BFA. D-F, H4 cells were treated with DMSO or AG1478 for different concentrations, different time, and then stained with an antibody against GM130. The images were analyzed by CellProfiler software. Bar, 10 μm.
