FIGURE 2.

The effect of AG1478 on Golgi is independent of EGFR. A, H4 cells were pretreated with AG1478 for 30 min, then treated with 50 ng/ml EGF for 5 min and analyzed for anti-phospho-EGFR (tyr1068) or tubulin by immunoblot. B, control Western blot for the effect of EGFR siRNA. Anti-tubulin was used as a loading control. C-F, H4 cells were co-transfected pEGFP-N1 plasmid with EGFR siRNA at 1:10 ratio for 24 h and then treated with DMSO or 14 μm AG1478 as indicated for 1 h. H4 cells expressing EGFP siRNA (D and F) were stained with anti-GM130 antibody (C and E). GFP-positive transfected cells are indicated by white asterisks. Bar, 10 μm.