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Rat2 cells are resistant to AG1478 but not BFA. A, Rat2 cells were treated with DMSO, 140 μm AG1478, or 5 μm BFA as indicated for 4 h, stained with anti-GM130 antibody. B, H4 cells or Rat2 cells were pretreated with 14 μm AG1478 for 30 min or not, then stimulated by 50 ng/ml EGF for 5 min, and analyzed by Western blotting using anti-phospho-EGFR (tyr1068), anti-phospho-ERK, and anti-tubulin antibody. Bar, 10 μm.
