Effects of AG1478 on the distribution of intracellular Golgi-associated
proteins. H4 cells expressing ARF1-GFP (A), Sec13-GFP
(D), p58-YFP (E), and GalT-CFP (G) were incubated
with DMSO, 14 μm AG1478, 5 μm BFA for 1 h, or H4
cells were incubated with DMSO, 14 μm AG1478, 5 μm
BFA for 1 h and then stained with antibodies against β-COP (B),
GBF1 (C), GM130 (F), p230 (H), adaptin γ
(I), adaptin δ (J), and LAMP3 (L).
K, H4 cells incubated with Alexa 568-labeled transferrin for 5 min
and then treated with DMSO, 14 μm AG1478, 5 μm BFA
for 1 h. Bar, 10 μm.