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. 2008 Nov 7;283(45):31087–31096. doi: 10.1074/jbc.M806592200

FIGURE 7.

FIGURE 7.

The effect of AG1478 on the GBF1-1 protein. A, schematic representation of different deletion constructs from GBF1-GFP. B and C, H4 cells were transfected with GBF1-1-GFP or GBF1-2-GFP for 24 h and then treated with DMSO, 14 μm AG1478, or 5 μm BFA as indicated for 1 h. D, H4 cells were transfected with GBF1-2-GFP for 24 h and treated with DMSO; treated with 14 μm AG1478 for 1 h; pretreated with 14 μm AG1478 for 1 h and then treated with 100 μm H89 together with AG1478 for 10 min; treated with 5 μm BFA together with AG1478 for 1 h; or pretreated with 5 μm BFA for 1 h and then treated with 14 μm AG1478 together with BFA for 1 h. Bar, 10 μm.