The effect of AG1478 on the GBF1-1 protein. A, schematic
representation of different deletion constructs from GBF1-GFP. B and
C, H4 cells were transfected with GBF1-1-GFP or GBF1-2-GFP for 24 h
and then treated with DMSO, 14 μm AG1478, or 5 μm
BFA as indicated for 1 h. D, H4 cells were transfected with
GBF1-2-GFP for 24 h and treated with DMSO; treated with 14 μm
AG1478 for 1 h; pretreated with 14 μm AG1478 for 1 h and then
treated with 100 μm H89 together with AG1478 for 10 min; treated
with 5 μm BFA together with AG1478 for 1 h; or pretreated with 5
μm BFA for 1 h and then treated with 14 μm AG1478
together with BFA for 1 h. Bar, 10 μm.