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. 2008 Nov 7;283(45):31005–31011. doi: 10.1074/jbc.M804464200

FIGURE 3.

FIGURE 3.

RSDP impairs GABAB receptor-mediated inhibition of spontaneous glutamate release in dissociated hippocampal neurons in culture. A, the percentage of inhibition of the mEPSC frequency by baclofen (100 μm) was assessed in individual neurons under control condition (ACSF, n = 5) and after incubation with 4 nm (n = 5) or 40 nm (n = 7) RSDP for the times indicated (for values, see supplemental Table S1). B, time course of the RSDP effect on the baclofen-induced mEPSC frequency inhibition in individual neurons (n = 5 per condition). C, summary histograms illustrating that incubation with 40 nm RSDP for 1 h impairs baclofen (bac)-but not adenosine (adeno)-mediated mEPSC frequency inhibition. Values are means ± S.E. of the percentage of inhibition of the mEPSC frequency (100 μm baclofen, ACSF, 88.6 ± 2.4% n = 5; RSDP, 15.7 ± 3.2%, n = 5, ***, p < 0.001, Kolmogorov-Smirnov; 100 μm adenosine, ACSF, 70.2 ± 4.0%, n = 5; RSDP, 78.6 ± 2.7%, n = 5). D, representative mEPSC recordings under baseline conditions, during adenosine application, after washing with ACSF (wash), during baclofen application, and after antagonizing GABAB receptors with CGP54626. Recordings from one cell each incubated with ACSF or RSDP are shown.