FIGURE 3.

C/EBPβ protein accumulates to a greater degree in cells expressing a C/EBPβ mRNA that does not bind HuR. Upper panel, total cell lysates were prepared from cells transduced with the indicated construct, and 25 μg of total protein was subjected to Western blot analysis for C/EBPβ. Molecular masses are indicated in kDa. CRM indicates cross-reacting material observed in this analysis. Lower panel, the blot was stripped and reprobed with β-tubulin, which served as a loading control. The experiment was performed twice with identical results.