FIGURE 4.

C/EBPα is expressed to a greater degree in the βd/s cells. At 8 days post-induction of differentiation, cell extracts were prepared, and 25 μg of protein of the three cell lines indicated was subjected to Western blot analysis for C/EBPα, C/EBPβ, and PPARγ. Molecular masses are indicated in kDa. B–D are from the same plot probed sequentially for C/EBPβ, PPARγ, and β-tubulin. A is from a separate Western blot. The experiment was performed twice with identical results.