FIGURE 1.

The L148S mutation does not alter the expression, localization, or ligand-binding properties of hGPR54. A, immunoprecipitation of WT and L148S hGPR54-GFP. B, confocal microscopy showing the localization of WT and L148S hGPR54-GFP. C, cell surface expression levels of WT and L148S HA-hGPR54 measured using fluorescence-activated cell sorting analysis. D, saturation binding of ¹²⁵I-labeled KP-10 to membranes from WT and L148S hGPR54 expressing HEK293 cells. The results presented in A and B are representative of three experiments performed in duplicate, and C and D show the means ± S.E. of three or four experiments performed in triplicate. IP, immunoprecipitation; IB, immunoblot.