FIGURE 2.

Pretreatment of BKV with HD5 inhibits infection. Purified BKV at the indicated m.o.i. was incubated with 50 μg/ml HD5 or the same volume of H₂O for 1 h at 4 °C in MEM containing 5% FBS, serum-free MEM, or PBS. Vero cells were then infected with the HD5/BKV mixture at 37 °C for 1 h. Unbound virus was removed by washing and complete media was added to cells. Cells were fixed and the percentage of the total cells expressing V-Ag determined at 72 h post-infection. The graph shows the average of five experiments. Error bars represent the standard deviation.