FIGURE 5.
Effect of preincubation of ALDH2 with GTN on acetaldehyde dehydrogenation. The figure shows the effects of preincubation of ALDH2 at pH 7.5 with 1 μm GTN on the subsequent determination of dehydrogenase activity in the presence of NAD+ at pH 7.4 in the absence and presence of 2 mm DTT and LPA-H2. Experimental conditions for preincubation were: 0.27 mg/ml ALDH2, 1 μm GTN, 0.2 mm MgCl2, 2 mm NAD+, 50 mm NaPi (pH 7.5) for 10 min at 37 °C. Assay conditions were: 1.5 μg/ml ALDH2, 2 mm NAD+, 10 mm MgCl2, and 0.2 mm acetaldehyde in 50 mm NaPi (pH 7.5) at 25 °C. See “Experimental Procedures” for further details.