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. 2008 Nov 7;283(45):30658–30667. doi: 10.1074/jbc.M800510200

FIGURE 1.

FIGURE 1.

Changes in mitochondrial gene expression during 3T3-L1 adipocyte differentiation. A, “All probes” refers to those detected with the MOE430-2 Affymetrix GeneChip, with a signal > 50. “Mito Probes” refers to probes annotated with gene ontology cellular component term “mitochondrion” retrieved directly from the Affymetrix website. Plotted are the average of all -fold increases and decreases in probe signal between the days of differentiation indicated on the abscissa. B, total DNA was extracted at the days indicated on the abscissa. Mitochondrial DNA (mtDNA) copy number was determined by qRT-PCR taking nuclear DNA as internal control and expressed as -fold change over day 0 of differentiation. C, the uptake of 2-deoxyglucose was measured after 30 min of incubation with the indicated concentrations of insulin at the times of differentiation indicated. Points represent means, and lines ±S.E. of two independent experiments performed in triplicate. D, Tfam mRNA expression determined by qRT-PCR, using ferritin mRNA as internal control and expressed as -fold change over day 0 of differentiation.