Changes in mitochondrial gene expression during 3T3-L1 adipocyte
differentiation. A, “All probes” refers to
those detected with the MOE430-2 Affymetrix GeneChip, with a signal > 50.
“Mito Probes” refers to probes annotated with gene
ontology cellular component term “mitochondrion” retrieved
directly from the Affymetrix website. Plotted are the average of all -fold
increases and decreases in probe signal between the days of differentiation
indicated on the abscissa. B, total DNA was extracted at the days
indicated on the abscissa. Mitochondrial DNA (mtDNA) copy number was
determined by qRT-PCR taking nuclear DNA as internal control and expressed as
-fold change over day 0 of differentiation. C, the uptake of
2-deoxyglucose was measured after 30 min of incubation with the indicated
concentrations of insulin at the times of differentiation indicated.
Points represent means, and lines ±S.E. of two
independent experiments performed in triplicate. D, Tfam mRNA
expression determined by qRT-PCR, using ferritin mRNA as internal control and
expressed as -fold change over day 0 of differentiation.