FIGURE 3.

Alignment of vertebrate Group I and II and invertebrate CTD sequences. Group I, Group II, and invertebrate CTD sequences were aligned using ClustalW and are displayed in Jalview, a multiple alignment editor that identifies conserved residues (blue, top rows); assesses “quality” of conservation (middle rows) by analyzing the proportion of identical conservative changes and non-conservation (scaled high (yellow), intermediate (brown), and low (black)); and defines an overall “consensus” (bottom rows). Islands of high sequence conservation (Domains I and II) and the PPPY sequence are indicated by boxes.