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. 2008 Oct 23;1:8. doi: 10.3389/neuro.02.008.2008

Figure 2.

Figure 2

Specificity of antibodies against DPP6 proteins. (A) DPP6 antibodies detected a band of approximately 115 kD in immunoblots of extracts from CHO cells transfected with DPP6-S (lane 1) or DPP6-L (lane 3). The antibodies were specific for detection of DPP6 proteins, since no bands were detected in extracts from non-transfected cells (lane 2) or cells transfected with DPP10 (lane 4). All cells were also transfected with GFP to control for transfection efficiency. (B) DPP6 antibodies detected a band of similar size in immunoblots of brain membranes obtained from wild type mice (lane 1) but not from DPP6 knockout mice (lane 2). (C) Immunofluorescent detection of DPP6 protein in DPP6-transfected CHO cells (left panel). No labeling is observed in untransfected CHO cells (right panel). Note that, as previously reported, transfection with DPPs produces flattening of CHO cells (Nadal et al., 2003; Zagha et al., 2005). (D) DPP6 antibodies labeled several brain structures in wild type mice. Immunoperoxidase staining was reduced to background levels in DPP6 knockout mice. Scale bar: 10 μm (C); 2 mm (D).