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Sensitivity of RVFV LAMP and real-time Taqman PCR (8). RVFV RNA extracted from supernatants of infected cells was serially diluted, and samples containing 105 to 10−2 copies were assayed in parallel using Taqman RT-PCR and our RT-LAMP assay. Real-time follow-up on a LightCycler (using channel F2/F3) and melting curve analysis for LAMP are reported. Reaction products were analyzed on agarose gels with ethidium bromide staining.
