TABLE 3.
Comparison of RVFV Taqman CT and LAMP times for detection using real-time follow-upa
| Estimated no. of RNA copies |
CT
|
|
|---|---|---|
| Taqman RT-PCR (no. of cycles) | RT-LAMP (min) | |
| 100,000 (105) | 18.2 | 7.9 |
| 10,000 (104) | 20.3 | 8.5 |
| 1,000 (103) | 27.4 | 10.5 |
| 100 (102) | 33.4 | 12.4 |
| 10 (101) | 39.9 | 18.0 |
| ∼1 (10°) | − | − |
| ∼0.1 (10−1) | − | − |
| ∼0.01 (10−2) | − | − |
a
RT-PCR Taqman and RT-LAMP were performed with a LightCycler instrument following procedures described in Materials and Methods. The CT was determined by the fit points method at the end of the run. Note that for the Taqman PCR, the difference from CT to CT between dilutions is not 3, since efficiency of the reaction is not 2 (slope is −4 instead of −3,3) (8).