. 2008 Sep 3;46(11):3595–3598. doi: 10.1128/JCM.01635-08

TABLE 1.

Sequences of primers used in PCRs for detection of BKV, JCV, and SV40, KIPyV and WUPyV, and MCPyV

Primer name	Sequence (5′-3′)	Virus	Region	Position (bp)	GenBank accession no.
Nested outer primers^a
P-3	GTATACACAGCAAAGGAAGC	BKV	LT	2475-2494	V01109 (strain MM)
		JCV	LT	4179-4198	NC_001699 (strain Mad-1)^b
		SV40	LT	4327-4346	AF316139 (strain 776)
P-4	GCTCATCAGCCTGATTTTGG	BKV	LT	2827-2846
		JCV	LT	4790-4809
		SV40	LT	4940-4959
Nested inner primers^c
P-1	AGTCTTTAGGGTCTTCTACC	BKV	LT	2551-2570	V01109 (strain MM)
		JCV	LT	4255-4274	NC_001699 (strain Mad-1)^b
		SV40	LT	4406-4425	AF316139 (strain 776)
P-2	GGTGCCAACCTATGGAACAG	BKV	LT	2707-2726
		JCV	LT	4408-4427
		SV40	LT	4453-4572
KIPyV2263.F	TTGGATGAAAATGGCATTGG	KIPyV	VP1	2263-2282	EF127906
		WUPyV	VP1	2411-2430	EF444550
KIPyV2404.R	TAACCCTTCTTTGTCTAAAATGTAGCC^d	KIPyV	VP1	2404-2378
		WUPyV	VP1	2552-2526
MCPyLT1709.F	CAGGCATGCCTGTGAATTAGGATG	MCPyV	LT	1709-1732	EU375803
MCPyLT1846.R	TCAGGCATCTTATTCACTCC	MCPyV	LT	1846-1827

First described by Bogdanovic et al., 1994 (5).

From reference 9a.

First described by Arthur et al., 1989 (2a).

The site of mismatch with the WUPyV sequence is boldfaced.