Figure 2.

DLP1 mutants do not affect endocytosis. Clone 9 cells expressing GFP vector alone, WT, K38A, or D231N were incubated with Texas Red–conjugated dextran or DiI-LDL, fixed, and observed by fluorescence microscopy. Cells containing fluorescent marker in the cytoplasm were counted positive for marker uptake. Quantitation of transfected cells reveals that DLP1 mutants do not affect the endocytosis of dextran (white bars) or LDL (gray bars) relative to cells expressing GFP vector alone. Time-course experiments in transfected cells with the use of Alexa-conjugated transferrin showed that DLP1 mutant–expressing cells were indistinguishable from untransfected cells at 2, 5, 10, or 20 min (our unpublished results). Data are expressed as percentage uptake normalized to cells expressing GFP vector alone.