Figure 8.

Ultrastructural quantitation of ER and mitochondrial phenotypes in GFP-DLP1-K38A–injected cells. ER and mitochondrial profiles were traced as depicted in Figure 7, and total ER and mitochondrial volume densities were calculated for five control and five K38A-expressing cells, as described in MATERIALS AND METHODS. The data are expressed as a percentage of total cell volume density ± SEM. In mutant cells, total mitochondrial volume density (gray bars) increased 43% over control. Strikingly, mutant cells exhibited an 80% decrease in the total ER volume density (stippled bars).