TABLE 2.

Performance and work-flow characteristics of Streptococcus agalactiae real-time PCR utilizing experimental carrot broth- and LIM broth-based enhancement protocols

PCR and protocol	Sensitivity (%)	Specificity (%)	Positive predictive value (%)	Negative predictive value (%)	Rate of unresolved results (%)
					Initial	Final
Carrot broth-enhanced PCR
Protocol A	100	99.3	98.5	100	0	0.0
Protocol B	98.5	99.3	98.5	99.3	10.3	3.0
LIM broth-enhanced PCR
Protocol A	92.5	99.3	98.4	96.4	0.5	0.5
Protocol B	92.5	97.8	95.4	96.4	1	0.0