TABLE 1.
Comparison of incubation and turnaround times for the manual and automated detection protocols
| Protocol (no. of tests) | Step | Time (min)a | Incubation temp (°C) |
|---|---|---|---|
| Manual (24) | Hybridization | 30 | 53 |
| Ambient wash | 1 | ||
| Stringent wash | 15 | 53 | |
| Conjugate | 30 | ||
| Ambient wash | 1 | ||
| Ambient wash | 10 | ||
| Ambient wash | 10 | ||
| Ambient wash | 5 | ||
| Citrate | 5 | ||
| Substrate | 10 | ||
| Distilled water | 5 | ||
| Distilled water | 5 | ||
| Distilled water | 0 | ||
| Incubation time | 127 | ||
| Turnaround timeb | 180 (approx) | ||
| BeeBlot (48)c | Preheat | 15d | 51.5 |
| Hybridization | 30 | 51.5 | |
| Ambient wash | 0 | ||
| Conjugate | 15 | ||
| Stringent wash | 12 | 51.5 | |
| Ambient wash aspirate | 0 | ||
| Ambient wash dispense | 5 | ||
| Ambient wash | 5 | ||
| Citrate | 5 | ||
| Substrate | 10 | ||
| Distilled water | 0 | ||
| Distilled water | 0 | ||
| Distilled water | 0 | ||
| Incubation time | 97 | ||
| Turnaround time | 150 (approx) |
a
Buffer preparation and strip labeling times are similar for both methods.
b
Turnaround time includes additional hands-on and/or instrument processing times.
c
Times and incubation temperatures as entered into the BeeBlot instrument.
d
Strips can be labeled during the preheat incubation.