Fig. 6.

Methylglyoxal viability assays in wild-type S. cerevisiae yeast, in the S. cerevisiae glyoxalase I mutant transformed with the pYES2 vector (Δglo1(pYES2)), and in the S. cerevisiae glyoxalase I mutant transformed with the pYES2:HC205 recombinant vector (Δglo1(pYES2:HC205)), grown in triplicate (1–3). The addition (+) or absence (–) of glucose which suppresses expression from the pYES2 vector is indicated in the left hand side panel. Galactose was added to the medium in the absence of glucose to activate expression of the pYES2 vector. Concentration of methylglyoxal added to the growth media is indicated in the right hand side panel. (This figure is available in colour at JXB online.)