Table 1.

RT-PCR primers used to identify mRNA transcripts for HC205/At1g07645, 18S rRNA, Arabidopsis LEA-2 (At1g76180), and Arabidopsis polyubiquitin 10 (At4g05320)

Gene name	Forward primer	Reverse primer	Annealing temperature	No. cycles
HC205/At1g07645	5′-ACAGGTGGGG(AG)GAGCT(AG)GAG-3′	5′-ATTAACGTGGCTTCCGA(TG)GCG-3′	58 °C	25
18S rRNA	5′-CAGGCGCGCAAATTACCCAA-3′	5′-GCGACCATACTCCCCCGG-3′	54 °C	20
LEA 2	5′-GTACAAAGAGCGTGAAATCCGC-3′	5′-CTTCTCCTCTACGGACC-3′	56 °C	30
Ubiqutin	5′-CAATTCTCTCTACCGTGA-3′	5′-CCATCTTCAAGTTGCTTTCCG-3′	55 °C	32

Degenerate nucleotides are shown in parenthesis. The annealing temperatures and number of cycles used in the PCR reactions are summarized for each set of primers.