FIG. 1.

Genomic location of YE3614 and sequence alignment of the GDSL lipase, SseJ, from S. enterica, and YE3614 from Y. enterocolitica JB580v. (A) Chromosomal location of YE3614 and its surrounding ORF. YE3610, homology to a lipoprotein; YE3610A, homology to a flagellar protein, pseudogene; YE3611, homology to a regulator, pseudogene; YE3614, homology to a type III secreted protein; YE3614A, homology to a flagella regulator, pseudogene; IS1330, disrupted ORF with homology to the IS10-like transposase from Y. enterocolitica. Gray arrows indicate ORFs with homology to ancestral flagellum cluster. (B) An amino acid alignment of YE3614 with SseJ from S. enterica. The five blocks of amino acid identity found in GDSL lipases are underlined. Arrowheads indicate catalytic residues (Ser, Asp, and His), black boxes indicate identical amino acids, and gray boxes indicate similar amino acids.