FIG. 3.

YspM is translocated into CHO cells by the Ysa T3SS. pSW43 (JB580v yspM ORF and promoter) was transformed into the wild-type (WT; JB580v), pYVe8081v-cured (JB580c), and ΔyscC (YVM1374) strains. Strains were used to infect CHO cells in triplicate at 26°C for 2 h. Cytosolic levels of cAMP were measured as described in Materials and Methods and compared to uninfected CHO cells and CHO cells infected with JB580v carrying the negative control plasmid, pSW42 (JB580v yspM promoter). Results of a representative assay are shown.