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Effect of 2-oxoglutarate on binding of NtcA to DNA fragments containing the ntcA promoter region bearing mutations in the proximal (A) or distal (B) NtcA-binding sites. The percentage of fragment retarded (one band in panel A and two bands in panel B) in parallel assays carried out in the absence (open circles) or presence (closed circles) of 2-oxoglutarate (see Fig. S1 in the supplemental material) is represented with respect to the NtcA concentration in the assays.
