TABLE 1.
Bacterial strains, plasmids, and phage used in this study
| Strain, plasmid, or phage | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | λ− φ80lacZΔM15 Δ(lacZYA-argF)U196 recA1 endA1 hsdR17 (rK− mK−) supE44 thi-1 gyrA relA1 | Stratagene |
| E. chrysanthemi strains | ||
| 3937 | Wild-type strain isolated from S. ionantha | |
| A3953 | pecS::MudIIPR13 Cmr | 49 |
| A4587 | nipE-uidA-Kmr | This work |
| A4921 | pecS::MudIIPR13 CmrnipE-uidA-Kmr | This work |
| A4961 | ahpC-uidA-Kmr | This work |
| A4987 | pecS::MudIIPR13 CmrahpC-uidA-Kmr | This work |
| A4952 | virK-uidA-Kmr | This work |
| A4978 | pecS::MudIIPR13 CmrvirK-uidA-Kmr | This work |
| A4946 | rhlA-uidA-Kmr | This work |
| A4979 | pecS::MudIIPR13 CmrrhlA-uidA-Kmr | This work |
| A4889 | ΔavrLM-uidA-Kmr | This work |
| A4920 | pecS::MudIIPR13 Cmr ΔavrLM-uidA-Kmr | This work |
| A5009 | fliC-uiDA-Kmr | V. Shevchik, personal communication |
| A3997 | prtE-uidA-Kmr | 26 |
| Ech176 | prtE::MudII1734 Kmr | F. Van Gijsegem, unpublished data |
| A4111 | vfmE::Cmr | S. Reverchon, unpublished data |
| A4496 | vfmE-uiDA-Kmr | S. Reverchon, unpublished data |
| Plasmids | ||
| pGEM-T | AprlacZ′ | Promega |
| pUIDK1 | pBR322 derivative harboring the uidA-Kmr cassette | 2 |
| pSC3154 | pGEM-T carrying the nipE gene | This work |
| pSR3323 | pGEM-T carrying the rhlA gene | This work |
| pSR3326 | pGEM-T carrying the virK gene | This work |
| pSR3329 | pGEM-T carrying the ahpC gene | This work |
| pSLF3 | pUC18 carrying the border regions flanking the avrLM gene cluster | This work |
| pSR3333 | pGEM-T carrying the nipE regulatory region | This work |
| pSR3325 | pGEM-T carrying the rhlA regulatory region | This work |
| pSR3328 | pGEM-T carrying the virK regulatory region | This work |
| pSR3332 | pGEM-T carrying the ahpC regulatory region | This work |
| pSR3334 | pGEM-T carrying the avrL regulatory region | This work |
| pSR1802 | pBluescript carrying the celZ regulatory region | 44 |
| Phage ΦEC2 | E. chrysanthemi generalized transducing phage | 47 |