TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotypes or relevant characteristic(s)a | Reference or source |
|---|---|---|
| S. aureus | ||
| 8325-4 | 8325 strain cured of three prophages | 10 |
| RN4220 | Restriction-deficient transformation recipient | 14 |
| RN6390 | Laboratory strain related to 8325-4 | 18 |
| SH1000 | Functional rsbU+ derivative of 8325-4 | 9 |
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17(rK− mK−) supE44 λ− thi-1 gyrA96 relA1 | Gibco-BRL |
| BL21 | E. coli B F−dcm ompT hsdSB(rB− mB−) gal λ | Stratagene |
| Plasmids | ||
| pSK950 | 10.5-kb plasmid carrying the attP site of phage L54a, replicon of pE194; Tcr Emr (S. aureus) | 20 |
| pQT5 | pTrcHisA-mgrA | 26 |
| pQT16 | pknB gene cloned into pSK950 | This study |
| pWN2018 | 10.5-kb S. aureus transcriptional promoter probe vector; Cmr | 25, 27 |
| pBF8-30 | 315-bp sequence containing the entire promoter of norA cloned upstream of the blaZ gene of pWN2018 | 25 |
| pLZ113 | E. coli-S. aureus shuttle vector pRB373 with xyl/tet promoter cloned into the MCS | 29 |
| pLZ-mgrA | pLZ113-mgrA | This study |
a
MCS, multiple cloning site.