TABLE 3.
Effect of pknB expression on levels of expression of norA and mgrA
| S. aureus strain(plasmid)a | Genotype expression levelb | Relative transcript levelc
|
||
|---|---|---|---|---|
| norA | mgrA | pknB | ||
| RN6390 | rsbU− | 1 | 1 | 1 |
| SH1000 | rsbU+ | 1.3 | 1 | 1.7 |
| RN6390(pSK950) | rsbU− | 1 | 1 | 1 |
| SH1000(pSK950) | rsbU+ | 1.3 | 1 | 1.7 |
| RN6390(pQT16) | rsbU−; pknB+++ | 5 | 1 | 8 |
| SH1000(pQT16) | rsbU+; pknB+++ | 5 | 1 | 8 |
a
Strains harboring plasmid pSK950 or pQT16 were grown in the presence of tetracycline (5 μg/ml).
b
−, negative; +, positive; +++, overexpression.
c
Values represent transcript (n-fold) changes relative to that of RN6390.