TABLE 1.
E. coli strains used in this studya
| Strain | Genotype or description | Source or reference |
|---|---|---|
| MA249 | ilv metB his-29 trpA9605 pro thyA deoB (or deoC) gyrB221(Cour) gyrB203(Ts) zie-3163::Tn10kan | 14 |
| MA251 | ilv metB his-29 trpA9605 pro thyA deoB (or deoC) gyrB221(Cour) gyrB203(Ts) zie-3163::Tn10kan topA20::Tn10 | 14 |
| IB34 | MA251(pSK760) | This study |
| RFM445 | rpsL galK2 gyrB221(Cour) gyrB203(Ts) Δlac74 | 11 |
| RFM475 | rpsL galK2 gyrB221(Cour) gyrB203(Ts) Δ(topA cysB)204 Δlac74 | 11 |
| VU4 | RFM475(pMD306) | This study |
| VU7 | VU4(pEM001) | This study |
| VU8 | VU4(pEM003) | This study |
| PS63 | RFM475 φ(lacIq-Ptrc-lac) | This study |
| PS64 | RFM445 φ(lacIq-Ptrc-lac) | This study |
| PS66 | PS63(pEM001) | This study |
| PS68 | PS63(pEM003) | This study |
| PS70 | PS64(pEM001) | This study |
| PS72 | PS64(pEM003) | This study |
| PS123 | PS63 rneΔ\14 | This study |
| PS126 | PS64 rneΔ14 | This study |
a
Cour, coumermycin resistance.