FIG. 4.

Phenotypic characterization of CdgG in the rugose genetic background. (A) Colony morphologies of rugose and RΔcdgG strains that were grown for 24 and 48 h on LB agar plates at 30°C. (B) Three-dimensional biofilm structures of rugose and RΔcdgG strains that are formed 8 h postinoculation under static conditions at 30°C. Images were acquired with CLSM, with top-down (large panes) and orthogonal (side panels) views of biofilms shown. Scale bar, 30 μm. (C) Transcription of vpsL-lacZ, vpsR-lacZ, and vpsT-lacZ fusions determined in rugose and RΔcdgG strains by measuring β-galactosidase activity in cells that were grown to mid-exponential phase in LB medium at 30°C. The results shown are representative of three independent experiments. Error bars represent the standard deviations. (D) Diameter of migration zone of rugose, RΔcdgG, RΔvps-I, and RΔflaA strains measured in LB soft agar plates (0.3%) after 18 h of incubation at 30°C. The data shown are representative of three independent experiments. Error bars represent the standard deviations.