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Analysis of rpoS mRNA longevity in a V. cholerae Δhns mutant. Strains C7258ΔlacZ (wild type [WT]) and AJB80 (C7258ΔlacZ Δhns::Km) were grown to an OD600 of 1.5 at 37°C; rifampin was added to block transcription; and samples were collected at different time points for RNA extraction and rpoS mRNA analysis as described in Materials and Methods.
