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Analysis of perA transcription as a measure of RpoS expression in V. cholerae Δhns and Δcrp mutants. Strains C7258ΔlacZ, AJB80 (C7258ΔlacZ Δhns::Km), WL7258ΔlacZ (Δcrp), and AJB82 (ΔlacZ Δhns::Km Δcrp) were grown to stationary phase (16 h) in TSB at 37°C. Total RNA was extracted, and the abundance of perA mRNA was determined by qRT-PCR. Each value is the average for three independent cultures. Error bars, standard deviations.
