TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| S17-1λPir | F−recA hsdR RP4-2 (Tc::Mu) (Km::Tn7) lysogenized with λpir phage | 9 |
| Vibrio cholerae | ||
| C7258 | Wild type, El Tor biotype | Peru, 1991 |
| C7258ΔlacZ | C7258 lacZ deletion mutant | This study |
| AJB80 | C7258ΔlacZ Δhns::Km | This study |
| AJB50ΔlacZ | C7258ΔlacZ ΔrpoS | This study |
| AJB51ΔlacZ | C7258ΔlacZ ΔhapR | This study |
| WL7258ΔlacZ | C7258ΔlacZ Δcrp | This study |
| AJB81 | AJB80 ΔrpoS | This study |
| AJB82 | AJB80 Δcrp | This study |
| Plasmids | ||
| pHNS12 | 0.9-kb SacI-BamHI DNA fragment 5′ of the hns ORF amplified with primers Hns120 and Hns1018 in pUC19 | This study |
| pHNS34 | 0.8-kb BamHI-SphI DNA fragment 3′ of the hns ORF amplified with primers Hns1467 and Hns2289 in pUC19 | This study |
| pΔHNS | BamHI-SphI fragments from pHNS34 in pHNS12 | This study |
| pΔHNSK | 1.2-kb BamHI Kmr cassette from pUC4K in pΔHNS | This study |
| pCVDΔHNSK | SacI-SphI Δhns::Km fragment from pΔHNSK in pCVD442 | This study |
| pHNS | 0.4-kb BamHI-HindIII DNA fragment carrying the hns ORF amplified with primers Hns162 and HnsD2 in pTTQ18 | This study |
| pTTHNS90 | 0.3-kb BamHI-HindIII fragment encoding 90 amino acids of the H-NS N terminus amplified with primers Hns162 and Hns429 in pTTQ18 | This study |
| pTTRpoS | 1.0-kb EcoRI-HindIII fragment carrying rpoS amplified with primers RpoS489 and RpoS1511 in pTTQ18 | This study |
| pTT3 | XbaI-PstI DNA fragment containing the rrnB T1T2 transcription terminator in pUC19 | 7, 52 |
| pTT3Lux | 0.3-kb SphI-HindIII DNA fragment carrying the V. harveyi luxC promoter amplified with primers LuxC84 and LuxC434 in pTT3 | This study |
| pLux-LacZ | 0.5-kb KpnI-HindIII fragment containing rrnB T1T2 and the luxC promoter fused to a promoterless lacZ gene in pKRZ1 | This study |
| pTT3PerA | 0.9-kb SphI-HindIII fragment carrying perA amplified with primers PerA498 and PerA987 in pTT3 | This study |
| pPerA-LacZ | 1.1-kb KpnI-HindIII fragment containing rrnB T1T2 and the perA promoter fused to a promoterless lacZ gene in pKRZ1 | This study |
| pRpoSLac5 | A SphI-HindIII DNA fragment containing the rpoS promoter region was cloned between the rrnB T1T2 transcription terminator and a promoterless lacZ gene in pKRZ1 | 49 |
| pLacZ12 | 0.9-kb SacI-BamHI DNA fragment 5′ of the lacZ ORF amplified with primers LacZ6 and LacZ859 in pUC19 | This study |
| pLacZ34 | 0.8-kb BamHI-SphI DNA fragment 3′ of the lacZ ORF amplified with primers LacZ3236 and LacZ4029 in pUC19 | This study |
| pΔLacZ | 0.8-kb BamHI-SphI DNA fragment from pLacZ34 in pLacZ12 | This study |
| pCVDΔLacZ | 1.6-kb SacI-SphI fragment from pΔLacZ in pCVD442 | This study |