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. 2008 Sep 12;190(22):7335–7345. doi: 10.1128/JB.00360-08

TABLE 2.

Primers

Primer name Sequencea
Bfr146 5′-TTGATGAGATGAAACACGCTGACCA
Bfr431 5′-GCTTGCAGATAATTTTGGATACCCG
CheR377 5′-TAGAAGTGCAGCAAAAACGTCCGG
CheR587 5′-CCATTAAGTTTTGCGGACGGAAGT
Cyt835 5′-GCTGTGGATATCATGGGGCGTTTA
Cyt948 5′-TCGTTGGATGGCGGTAAAATGGGT
FlaA40 5′-GCACAACGTTATCTGACCAAAGGC
FlaA291 5′-GAGTTGGTACCGTTCGCCGATTG
FlaC405 5′-CAAGCTGCTCAACGGTACATTCG
FlaC592 5′-GCCTTCGCGTTTGTCTTTCAGAGT
Hns120 5′-GAAGAGCTCGAAGATGGTGAACGTA
Hns162 5′-GAAGGATCCGGAAATGGTAATGTCG
Hns429 5′-GGCCAAGCTTTTTCGTTTTAGTTTCG
Hns1018 5′-GCAGGATCCATGAAGGTAAATCTCT
Hns1467 5′-GATGGATCCTGACAAGTTTTCGCTG
Hns2289 5′-GATGCATGCCCCTCTTTGACAAACA
HnsD2 5′-GGGAAAGCTTGGCAAAATTACAGAG
KatB594 5′-TACCGAACAAGGCAACTGGGATTT
KatB792 5′-GTTCGATAGCTTGCCGGTGTACT
LacZ6 5′-GCGGATCCAGCCGAGGAGTAAAGA
LacZ859 5′-GCGAGCTCCGAAAATGACTGTTGT
LacZ3236 5′-GCGGATCCAAAGCAAGAGCCA
LacZ 4029 5′-GCGCGCATGCAACTCGGCTATCGTCC
LuxC84 5′-GCGCAAGCTTAATCGATTTTCTTCAGTAG
LuxC434 5′-GCGCGCATGCACACTGTCACACATC
MotX253 5′-GGAGTGTGTGTGGATCAGGATGTT
MotX374 5′-GGATCGCGCGTTCTTTGTCTTGTT
PerA498 5′-GTTGCATGCCTTTACCACCTTGATC
PerA607 5′-GAAAATAGCCGTTACTCTGGTCAGC
PerA863 5′-CTAAGCCTTGTTCGTGCAGTTCAG
PerA987 5′-GCGAAGCTTATTGATTACTCCTTGC
RecA578 5′-GTGCTGTGGATGTCATCGTTGTTG
RecA863 5′-CCACCACTTCTTCGCCTTCTTTGA
RpoS576 5′-CGATTTTGAAGATGAAGCACTGGAAG
RpoS489 5′-GTTGAATTCGAGGCCGCTATGAGT
RpoS1003 5′-TGTTTGGTTCATCAGCGCACGTTC
RpoS1511 5′-GCGAAGCTTTTTTGGATGAGTCTGG
ToxR369 5′-GGAAACGGTTGAAGAAGAGATGGC
ToxR498 5′-TTATTCGTCACAACATTGGCTGGC
a

Restriction sites used for directional cloning are underlined.