FIG. 3.
(A) ATP and prodigiosin during low-density growth in base complex broth. S. marcescens Nima was pregrown on a base complex medium slant at 26°C and washed into base complex broth to an initial OD750 of 0.12. Growth was continued with aeration. Samples were removed at 0.25-h intervals for assay of cell, ATP, and prodigiosin concentrations. Vertical arrows identify low density as 1.50 through 5.75 h. The vertical double line separates low-density phase 1 (1.50 to 3.00 h) from phase 2 (3.25 to 5.75 h). (B) ATP per cell and prodigiosin per cell during low-density growth in base complex broth. Data for ATP per cell and prodigiosin per cell from the experiment in panel A are presented.