TABLE 3.

Expected and actual cell yields after 56 h for Y. lipolytica grown under various culture conditions^a

Culture conditions	Substrate consumed (g liter−1)			Theoretical biomass provided by the nitrogen sources available (g liter−1)		Total theoretical biomass (g liter−1)	Theoretical biomass provided by the carbon sources available (g liter−1)			Total theoretical biomass (g liter−1)	Measured biomass (g liter−1)
	Amino acids	Ammonium citrate	Lactate	Amino acids	Ammonium citrate		Amino acids	Ammonium citrate	Lactate
Lactate		0.6	2.5		0.72	0.72		0.26	1.25	1.51	0.61b
0.1× amino acids	0.568	0.6		0.91	0.72	1.63	0.24	0.26		0.50	0.36c
0.1× amino acids + lactate	0.568	0.6	7	0.91	0.72	1.63	0.24	0.26	3.5	4	2.19b
2× amino acids	11.36	0.6		18.18	0.72	18.9	4.77	0.26		5.03	4.40c
2× amino acids + lactate	11.36	0.6	2	18.18	0.72	18.9	4.77	0.26	1	6.03	5.12c

^aThe yields for theoretical calculation of biomass were 10% for nitrogen and 50% for carbon.

^bNitrogen limitation.

^cCarbon limitation.