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. 2008 Sep 12;74(21):6774–6781. doi: 10.1128/AEM.01233-08

FIG. 2.

FIG. 2.

Heterogeneity of S. coelicolor microcolonies following germination. Numbers represent minutes after initiation of time-lapse microscopy (see Movies S2A, S2B, S2C, and S2D in the supplemental material). Horizontal white bars represent 10 μm. The four observed classes of S. coelicolor germination are displayed. (A) Class A germination, showing emergence of the primary hypha (1), growth arrest of the primary hypha (2) following branch emergence (3), and growth restoration of the primary hypha (4). (B) Class B germination, showing two hyphae emerging from the spore (1 and 2), growth arrest by one hypha (3), and growth restoration of the arrested hypha (4). (C) Class C germination, showing hyphal emergence (1) and branch appearance (2) (no growth arrest of the primary hypha). (D) Class D germination, showing emergence of the primary hypha (1) and emergence of the secondary hypha (2) from the spore (no growth arrest of the primary hypha).