TABLE 1.
Heterogeneity of S. coelicolor microcolonies following germination
| Germination classa | No. of hyphae emerging from each spore | Growth arrest of primary hypha | % of germinations | e2 − e1b (h) | e1 arrestc (h) |
|---|---|---|---|---|---|
| A | 1 | Yes | 14.3 | 2.7 (1.45) | 3.1 (2.16) |
| C | 1 | No | 49.0 | 5.7 (1.62) | |
| B | 2 | Yes | 20.4 | 1.4 (1.46) | 4.9 (2.44) |
| D | 2 | No | 16.3 | 3.5 (2.23) |
Germination of a random selection of spores was observed by time-lapse microscopy, and four classes of spore germinations were observed: one or two hyphae, with or without growth arrest.
Time difference between emergence of primary hypha at time e1 from a spore and appearance of a second extension site at time e2; the latter was the first hyphal branch (class A or C) or the emergence of a second hypha from the spore (class B or D). e2 − e1 values were compared statistically by the Student t test with respect to arrested (class A) and non arrested spores (class C) with one germ tube (P < 1 × 10−4), arrested (class B) and non arrested (class D) spores with two germ tubes (P < 0.013), arrested spores with one (class A) or two (class B) germ tubes (P < 0.048), and nonarrested germ tubes with one (class C) or two (class D) spores (P < 0.004). All four comparisons showed significant differences between the two populations in each individual test. Standard deviations are shown in parentheses.
Time between cessation of growth of primary hypha and subsequent restart of growth. Standard deviations are shown in parentheses.