TABLE 4.
The 23 CGF primers and the conditions for their amplification
| Primer | Expected size (bp) | Annealing temp (°C) | Forward primer (5′→3′) | Reverse primer (5′→3′) |
|---|---|---|---|---|
| fprn02 | 413 | 55.0 | ACGGGCAATCACTGAACTC | TGACGGCGATATAAGGACC |
| fprn30 | 614 | 55.0 | TCGCCATTACCAACTGAC | GATAACGGGCATCGACTC |
| fprn03 | 347 | 57.0 | CAATGTATCGCTGTACCGACTC | GCAGGACTGGACAATCACTAAG |
| fprn05 | 422 | 55.0 | GCCGGTCATCTTTCTCAC | GCGCATTTGCGTAACTCC |
| fprn10 | 408 | 55.0 | TTGAGGCCGAACGTAAAC | CGCCTCTTAACGGTAGTC |
| fprn11 | 442 | 55.0 | GCGGATGAATCCCTCAATCG | CCAGCAGGAACATCGCATAG |
| fprn13 | 208 | 55.0 | ACCGCCCTGGATATCTTC | ACGTCAGCTGGAGTCTTG |
| fprn28 | 954 | 57.0 | CCGCAGCCCATATCGAATAAGG | TCATGCGTCTCTGGGTTACTCC |
| fprn14 | 150 | 55.0 | CTCGTCCAGAAGGTCAATGATG | CCTCGCCGAGAGAAATTAAG |
| fprn15 | 648 | 55.0 | GGTCGCATGCCTTAGATAACTC | TTGGTGGTCCGATGCTTGAAG |
| fprn32 | 2210 | 55.0 | CGTTGCGGTTTGTGTATC | CTGCGGTAGTGAATAACG |
| fprn29 | 1002 | 55.0 | CCATCGTCAACCTCATC | GCTCCGTAAGTCAATGG |
| fprn33 | 359 | 55.0 | TGTCACCTCCCTACTATCAC | TGCCATGAGCGACATTG |
| fprn16 | 512 | 55.0 | GAAAGGTTAGCGCGGATG | TCAGCCAAACGTCTCTTC |
| fprn34 | 1007 | 55.0 | AACCGGGACATTAACGAC | ACGAAGAGCGAGAATGAG |
| fprn18 | 101 | 55.0 | TTCACGCCAAATGGCAAC | TCTGCAGGACCTATTGCAGTAG |
| fprn06 | 807 | 57.0 | ACCCGCTTTAGATTCTACCG | AAAGAGCGCAGTGATAAGCC |
| fprn07 | 926 | 57.0 | GCAGGACTGCCAGAAAGTTAAG | CGCCGATTTCCCTAAAGGTAAG |
| fprn35 | 489 | 55.0 | ACCAGCGCATGGAACTTG | GCGTCGCTCTTGAAACAC |
| fprn24 | 691 | 55.0 | CACTGGTTACCAGCTATG | CGCAAGCCTGTAAAGAAG |
| A01a | 432 | 60.8 | ACCAAGGCATCCCCCGTGTGAA | ATAATCCGCTGGGGCTGGCTGAC |
| B01a | 316 | 60.7 | AAGCCCCCACGTAATTCCCTGACA | CATTTCCCGCGCCTGACTGAGA |
| A12a | 446 | 55.9 | GGGCGGACTTTGTTTGGTTGAA | GCCTGGCGGAAATGGACTGTAT |
a
Identified by Steele et al. (48).