TABLE 1.
Phage susceptibility of L. monocytogenes isolates from turkey processing plants
| Isolate no. (alternative designation) | Date (mo/yr) | Source or reference | Genetic markersa | BCb | Phage susceptibility toc:
|
||
|---|---|---|---|---|---|---|---|
| 20422-1 | 805405-1 | A511 | |||||
| Serotype 4b (n = 17) | |||||||
| 1493 (L0226) | 08/03 | Overhead pipe, plant B (9) | ECII | − | + | + | + |
| 532 | 09/03 | Floor, plant A | ND | − | + | + | + |
| 1106 | 09/03 | Floor drain, plant A | ECII | − | + | + | + |
| 1117d (34-6a) | 09/03 | Floor drain, plant A (9) | ECII | + | − | − | − |
| 1495 (L0315) | 10/03 | Floor, plant B (9) | ECII | − | + | + | + |
| 1497 (L0328) | 10/03 | Air-conditioning unit, plant B (9) | ECI | + | + | + | + |
| 1277 (82-2a) | 12/03 | Floor drain, plant A (9) | ND | − | + | + | + |
| 1501 (L0617) | 02/04 | Raw product, plant B (9) | ECI | − | + | + | + |
| 1498 (L0603) | 02/04 | Dip tank, plant B (9) | ECII | − | + | + | + |
| 80 (171A) | 04/04 | Floor drain, plant A (9) | ND | − | + | + | + |
| 1506 (L0704) | 05/04 | Sink and hose, plant B (9) | ECII | − | + | + | + |
| 1513 (L0719) | 05/04 | Floor, plant B (9) | ECII | − | + | + | + |
| 900 | 10/04 | Floor drain, plant A | ND | − | + | + | + |
| 1157 | 04/05 | Floor drain, plant A | ND | − | + | + | + |
| 2509 | 03/06 | Cage dumper slide, plant D | ND | − | + | + | + |
| 2688 | 03/06 | Conveyor belt, plant D | ND | − | + | + | + |
| 2616 | 03/06 | Table, plant A | ND | − | + | + | + |
| Total susceptibility | 16/17 (94%) | 16/17 (94%) | 16/17 (94%) | ||||
| Serotype 1/2a or 3a (n = 24) | |||||||
| 175 | 09/03 | Drain, plant A | − | + | + | + | |
| 483 | 04/04 | Roast drain, plant A | + | − | − | − | |
| 513 | 04/04 | Floor, plant A | − | − | − | − | |
| 10 | 04/04 | Floor drain, plant D | + | + | + | + | |
| 90 | 06/04 | Chiller rework drain, plant A | − | − | − | − | |
| 93 | 06/04 | Chiller drain, plant A | + | + | + | − | |
| 162 | 06/04 | Floor drain, plant A | + | + | + | − | |
| 627 | 06/04 | Ground-meat floor, plant A | + | − | − | − | |
| 653 | 06/04 | Boots, plant A | + | − | − | − | |
| 720 | 08/04 | Floor drain, plant A | + | − | − | − | |
| 884 | 10/04 | Chiller drain, plant A | + | + | + | + | |
| 1559 | 10/04 | Floor fecal, plant F | − | + | + | + | |
| 1566 | 10/04 | Raw product, plant F | − | + | + | + | |
| 1096 | 12/04 | Ground-meat floor, plant A | + | − | − | − | |
| 1637 | 01/05 | Raw product, plant B | − | + | + | + | |
| 1747 | 03/05 | Cart wheels, plant C | − | + | + | + | |
| 1845 | 07/05 | Raw product, plant B | − | + | + | + | |
| 1907 | 06/05 | Drains, plant F | + | + | + | + | |
| 2622 | 03/06 | Bucket, plant A | + | − | − | − | |
| 2627 | 03/06 | Rehang drain, plant A | − | − | − | − | |
| 2506 | 03/06 | Cages (live birds), plant D | − | + | + | + | |
| 2507 | 03/06 | Cages (live birds), plant D | − | − | − | + | |
| 2508 | 03/06 | CO2 table, plant D | − | + | + | + | |
| 2662 | 03/06 | Carcass (before wash), plant D | − | + | + | + | |
| Total susceptibility | 14/24 (58%) | 14/24 (58%) | 13/24 (54%) | ||||
| Serotype 1/2b or 3b (n = 16) | |||||||
| 1491 | 08/03 | Cart, plant B | − | + | + | + | |
| 174 | 09/03 | Floor, plant A | − | − | − | − | |
| 1104 | 09/03 | Drain, plant A | + | − | − | − | |
| 1499 | 02/04 | Cart, plant B | − | − | − | − | |
| 83 | 04/04 | Roast drain, plant A | + | − | − | − | |
| 523 | 04/04 | Floor drain, plant A | − | − | − | − | |
| 27 | 04/04 | Chiller, plant D | − | + | + | − | |
| 1505 | 05/04 | Floor drain, plant B | − | − | − | − | |
| 1507 | 05/04 | Floor drain, plant B | − | − | − | − | |
| 597 | 06/04 | Floor drain, plant A | + | − | − | − | |
| 172 | 06/04 | Boots, plant A | + | − | − | − | |
| 706 | 08/04 | Table, plant A | − | − | − | − | |
| 731 | 08/04 | Rehang catwalk, plant A | − | + | + | − | |
| 854 | 10/04 | Live hang sink, plant A | + | + | + | − | |
| 197 | 12/04 | Floor drain, plant D | + | + | + | − | |
| 1830 | 05/05 | Raw product, plant B | − | + | + | + | |
| Total susceptibility | 6/16 (37%) | 6/16 (37%) | 2/16 (12.5%) | ||||
| Serotype 1/2c or 3c (n = 3) | |||||||
| 130d | 07/04 | Raw product, plant D | + | − | − | − | |
| 961 | 10/04 | Boots, plant A | + | − | − | − | |
| 2642 | 03/06 | Floor drain, plant A | + | − | − | − | |
| Total susceptibility | 0/3 (0%) | 0/3 (0%) | 0/3 (0%) | ||||
a
Genetic markers for ECI and ECII were determined as described previously (7, 9). ND; not determined.
b
Resistance to benzalkonium chloride (BC) was described previously (25). +, BC resistant; −, BC sensitive.
c
+, phage sensitive; −, phage resistant.
d
In these strains, infections with high-titer preparations of 20422-1 and 805405-1 occasionally resulted in very opaque plaques which were difficult to detect unambiguously and could not be accurately enumerated. For this reason, strains 1117 and 130 were designated as phage resistant.