TABLE 2.
Sequence percent similarity values within the methanogens for mcrAa
| Family | Mean % similarity | Genus | No. of isolates in analysis | Range of % similarity (mean) | GenBank accession no. |
|---|---|---|---|---|---|
| Methanobacteriaceae | 71.7 | Methanobacterium | 4 | 69.3-75.8 (73.1) | AF414050, AY386125, AF313806, AY289750 |
| Methanobrevibacter | 4 | 77.2-90.0 (82.6) | AF414046, AF414035, DQ251045, DQ251046 | ||
| Methanosphaera | 1 | NA | AF414047 | ||
| Methanothermobacter | 4 | 97.2-100.0 (98.6) | U10036, AY303950, AY289752, AY289748 | ||
| Methanothermaceae | 98.0 | Methanothermus | 2 | (98.0) | J03375, AY289747 |
| Methanococcaceae | 83.2 | Methanococcus | 4 | 76.0-92.7 (83.3) | M16893, AY354034, BX957223, X07793 |
| Methanothermococcus | 2 | 85.9 | AF414048, AY354033 | ||
| Methanocaldococcaceae | 81.4 | Methanocaldococcus | 2 | 82.5 | AF414040, AY354035 |
| Methanotorris | 1 | NA | AF414039 | ||
| Methanomicrobiaceae | 79.5 | Methanomicrobium | 1 | NA | AF414044 |
| Methanoculleus | 5 | 89.3-100.0 (92.1) | AF414036, AB288270, NZAASI01000002, AF313804, DQ229156 | ||
| Methanofollis | 1 | NA | AF414041 | ||
| Methanogenium | 4 | 82.8-93.8 (86.9) | DQ229157, DQ229158, DQ229159, DQ229160 | ||
| Methanocorpusculaceae | 94.9 | Methanocorpusculum | 3 | 93.8-97.1 (94.9) | AY260445, AF414049, AY260441 |
| Methanosarcinaceae | 79.4 | Methanosarcina | 7 | 88.3-99.3 (91.8) | Y10058, AE010299, AY260443, AF414043, U22248, U22250, U22251 |
| Methanococcoides | 2 | 96.0 | U22235, U22234 | ||
| Methanohalobium | 1 | NA | U22236 | ||
| Methanohalophilus | 3 | 95.0-99.0 (96.7) | U22237, U22259, U22239 | ||
| Methanolobus | 5 | 87.4-96.3 (91.6) | U22244, U22257, U22242, U22243, U22245 | ||
| Methanomethylovorans | 1 | NA | AY260442 | ||
| Methanosalsum | 1 | NA | U22252 | ||
| Methanosaetaceae | 79.6 | Methanosaeta | 2 | 79.6 | AF313802, CP000477 |
| Mean % similarity | 83.5 | 88.9 | |||
| With multiple genera | 79.0 |
a
Ranges and means were calculated using distance matrices created with the Jukes-Cantor algorithm. NA, not applicable.