FIG. 3.

Analysis of the inserted fragments of two 3′-RACE clones. First-strand cDNA was synthesized using an oligo(dT) anchor primer. Then, PCR was performed using the anchor primer and the Der f 7-specific primer, and amplified PCR fragments were cloned by insertion into the pCRII TA-cloning vector. The resulting plasmid DNAs were isolated and digested with EcoRI, followed by agarose gel electrophoresis to compare the lengths of the inserted fragments. The arrow indicates the bands of the TA-cloning vector used for cloning of the RT-PCR products, and the arrowhead indicates a 500-bp molecular marker.