TABLE 1.

Oligonucleotides used for total synthesis of the codon-optimized Der f 7 gene

Oligonucleotide no.	Sequence (5′→3′)
1	5′-CCCGTCGACCATGATGAAGTTCTTGCTGATCGCTGCCGTCGCCTTCGTCGCCGTTTCGGCTGACCCCATTCACTACGACAAGATCACCGAGGAAATCAACAAGGCTATCG-3′a
2	5′-ACGCTCGAACTTGTCGGCGTGGTCAGGGACCTTCATGGGATCGATGGTCTCGGACTGTTCGATAGCAGCAATGGCATCGTCGATAGCCTTGTTGATTT-3′
3	5′-CGACAAGTTCGAGCGTCACGTTGGTATCGTGGACTTCAAGGGTGAGTTGGCCATGCGCAACATCGAGGCTCGCGGCCTCAAGCAGATGAAGCGTCA-3′
4	5′-TCGACACGATATCATCGTGAACACCGATGAGCAAATGAGCCTTAACAATACCCTCTTCACCCTTGACATTAGCGTCACCCTGACGCTTCATCTGCTT-3′
5	5′-CGATGATATCGTGTCGATGGAGTACGATCTCGCCTACAAGCTGGGTGACCTTCATCCCACCACTCACGTCATCTCGGATATTCAGGACTTCGT-3′
6	5′-TGACAACATTAGCGAATTGGCGGACCTCGAAAGAGGTCATGGTGATGTTACCTTCGTCAGAAATCTCAAGGGAGAGGGCAACAACGAAGTCCTGAATATC-3′
7	5′-CAATTCGCTAATGTTGTCAACCACATCGGTGGCCTTTCCATCCTCGACCCCATTTTCGGCGTTCTCTCTGATGTCCTGACCGCTATCTTCCAAGACACCG-3′
8	5′-CCCTCTAGATTAATTCTTTTCCAGCTCACGCTTGAAGGCGGGGGCCAGGACCTTGGTCATTTCCTTACGGACGGTGTCTTGGAAGATAG-3′b

^aThe SalI site is underlined.

^bThe XbaI site is underlined.